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Mapping the structure-activity relationship of
Worawan PANPIPAT, Hasene KESKIN, Zheng GUO
《化学科学与工程前沿(英文)》 2015年 第9卷 第1期 页码 105-113 doi: 10.1007/s11705-015-1502-x
关键词: β-sitosteryl fatty acid esters dipalmitoyl phosphatidylcholine Langmuir monolayer condensing effect structure-activity relationship
Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao
《环境科学与工程前沿(英文)》 2016年 第10卷 第6期 doi: 10.1007/s11783-016-0884-4
关键词: Flow cytometry Escherichia coli Staphylococcusaureus UV CTC SYTO 9
Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic
Kim Yng Ooi, Jin Chuan Wu
《化学科学与工程前沿(英文)》 2015年 第9卷 第3期 页码 381-385 doi: 10.1007/s11705-015-1534-2
关键词: L-lactic acid thermophilic strain Bacillus coagulans dry yeast cells autolysis fermentation
血清<em>Nem>-聚糖生物标志物诊断ALT水平正常慢性乙型肝炎患者显著肝纤维化和肝硬化的临床意义 Article
王林, 刘艺琪, 顾启馨, 张驰, 徐蕾, 王蕾, 陈翠英, 刘学恩, 赵鸿, 庄辉
《工程(英文)》 2023年 第26卷 第7期 页码 151-158 doi: 10.1016/j.eng.2023.03.008
本研究目的是探讨血清<em>Nem>-聚糖模型在285例丙氨酸转移酶(alanine aminotransferase, ALT)水平正常应用基于DNA测序仪的荧光糖电泳技术检测患者血清<em>Nem>-聚糖图谱,每例患者的血清样本中共鉴定出9个<em>Nem>-聚糖峰。),并比较血清<em>Nem>-聚糖模型和其他纤维化标志物的诊断效能。在诊断肝硬化(≥F5)时,血清<em>Nem>-聚糖RF-B模型的AUROC为0.97,与肝组织活检的符合率为88.94%。在ALT水平正常的慢性乙肝患者中,血清<em>Nem>-聚糖模型可作为诊断显著肝纤维化或肝硬化的潜在生物标志物。
Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma
《医学前沿(英文)》 2023年 第17卷 第2期 页码 240-262 doi: 10.1007/s11684-022-0936-z
关键词: glioma progression molecular classification EM/PM subtyping intratumor heterogeneity
人体前列腺特异性抗原携带含酮基-脱氧壬酮糖酸的<em>Nem>-聚糖 Article
Wei Wang, Tao Zhang, Jan Nouta, Peter A. van Veelen, Noortje de Haan, Theo M. de Reijke, Manfred Wuhrer, Guinevere S.M. Lageveen-Kammeijer
《工程(英文)》 2023年 第26卷 第7期 页码 119-131 doi: 10.1016/j.eng.2023.02.009
Ketodeoxynononic acid (Kdn) is a rather uncommon class of sialic acid in mammals. However, associations have been found between elevated concentrations of free or conjugated Kdn in relation to human cancer progression. Hitherto, there has been a lack of conclusive evidence that Kdn occurs on (specific) human glycoproteins (conjugated Kdn). Here, we report for the first time that Kdn is expressed on prostate-specific antigen (PSA) N-linked glycans derived from human seminal plasma and urine. Interestingly, Kdn was found only in an α2,3-linkage configuration on an antennary galactose, indicating a highly specific biosynthesis. This unusual glycosylation feature was also identified in a urinary PSA cohort in relation to prostate cancer (PCa), although no differences were found between PCa and non-PCa patients. Further research is needed to investigate the occurrence, biosynthesis, biological role, and biomarker potential of both free and conjugated Kdn in humans.
结直肠癌、基质和正常结肠黏膜显微解剖区域<em>Nem>-糖组的显著多样性 Article
Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer
《工程(英文)》 2023年 第26卷 第7期 页码 32-43 doi: 10.1016/j.eng.2022.08.016
Aberrant glycosylation is considered to be a hallmark of colorectal cancer (CRC), as demonstrated by various studies. While the N-glycosylation of cell lines and serum has been widely examined, the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures. To overcome these obstacles, we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous, stromal, and healthy mucosa cells. N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray ionization tandem mass spectrometry, enabling the differentiation and structural characterization of isomeric species. In total, 116 N-glycans were identified that showed profound differences in expression among cancer, stroma, and normal mucosa. In comparison with healthy mucosa, the cancer cells showed an increase in α2-6 sialylation and monoantennary N-glycans, as well as a decrease in bisected N-glycans. Moreover, specific sialylated and (sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers. In comparison with cancer, the stroma showed lower levels of oligomannosidic and monoantennary N-glycans, LewisA/X epitopes, and sulfation, as well as increased expression of (core-)fucosylation and α2-3 sialylation. Our study reveals the distinct N-glycomic profiles of different cell types in CRC tumor and control tissues, proving the necessity of their separate analysis for the discovery of cancer-associated glycans.
关键词: 结直肠癌 肿瘤 多孔石墨化碳液相色谱-质谱 <em>Nem>-糖组 抗体反应
VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications
Mani Abirami, Krishnan Kannabiran
《化学科学与工程前沿(英文)》 2016年 第10卷 第4期 页码 542-551 doi: 10.1007/s11705-016-1599-6
关键词: Streptomyces ghanaensis VITHM1 nanoparticles 3D structure antibacterial activity
Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI
《环境科学与工程前沿(英文)》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0854-x
关键词: Photo-activated disinfection Titanium dioxide Nanowire membrane Silver Copper
可去除染料——<em>Nem>-聚糖多方法深入分析中的缺失环节 Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
《工程(英文)》 2023年 第26卷 第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
基于正交质谱的<em>Nem>-糖组谱揭示哈夫病潜在病原学 Article
刘思, 刘圆圆, 林佳静, 刘笔锋, 何振宇, 吴晓旻, 刘欣
《工程(英文)》 2023年 第26卷 第7期 页码 63-73 doi: 10.1016/j.eng.2022.09.012
Abiotic association of phthalic acid esters with humic acid of a sludge landfill
Xiaoli CHAI, Yongxia HAO, Xin ZHAO, Guixiang LIU, Ying ZHU, Rong JI, Jun WU, Huanhuan TONG, Youcai ZHAO
《环境科学与工程前沿(英文)》 2012年 第6卷 第6期 页码 778-783 doi: 10.1007/s11783-012-0434-7
关键词: abiotic association phthalic acid esters (PAEs) humic acid sludge landfill
Isolation and application of predatory
Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li
《环境科学与工程前沿(英文)》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0900-3
关键词: Bdellovibrio-and-like organisms (BALOs) Biolysis Activated sludge Dewaterability Predation
血清免疫球蛋白G <em>Nem>-糖基的高通量分析——一种消化道癌症的非侵入性生物标志物 Article
刘鹏程, 王小兵, 顿爱社, 李昱潼, 李厚强, 王璐, 张怡春, 李灿灿, 张金霞, 张晓雨, 马立兴, 侯海峰
《工程(英文)》 2023年 第26卷 第7期 页码 44-53 doi: 10.1016/j.eng.2023.02.008
Chunhao Dai, Pufeng Qin, Zhangwei Wang, Jian Chen, Xianshan Zhang, Si Luo
《环境科学与工程前沿(英文)》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0892-z
关键词: Open top chamber Gaseous elemental mercury (GEM) Soil Mercury Brassica napus
标题 作者 时间 类型 操作
Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid
Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao
期刊论文
Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic
Kim Yng Ooi, Jin Chuan Wu
期刊论文
人体前列腺特异性抗原携带含酮基-脱氧壬酮糖酸的<em>Nem>-聚糖
Wei Wang, Tao Zhang, Jan Nouta, Peter A. van Veelen, Noortje de Haan, Theo M. de Reijke, Manfred Wuhrer, Guinevere S.M. Lageveen-Kammeijer
期刊论文
结直肠癌、基质和正常结肠黏膜显微解剖区域<em>Nem>-糖组的显著多样性
Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer
期刊论文
VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications
Mani Abirami, Krishnan Kannabiran
期刊论文
Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI
期刊论文
可去除染料——<em>Nem>-聚糖多方法深入分析中的缺失环节
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
期刊论文
Abiotic association of phthalic acid esters with humic acid of a sludge landfill
Xiaoli CHAI, Yongxia HAO, Xin ZHAO, Guixiang LIU, Ying ZHU, Rong JI, Jun WU, Huanhuan TONG, Youcai ZHAO
期刊论文
血清免疫球蛋白G <em>Nem>-糖基的高通量分析——一种消化道癌症的非侵入性生物标志物
刘鹏程, 王小兵, 顿爱社, 李昱潼, 李厚强, 王璐, 张怡春, 李灿灿, 张金霞, 张晓雨, 马立兴, 侯海峰
期刊论文